Journal: PLoS ONE

Article Title: Bone marrow derived-mesenchymal stem cells downregulate IL17A dependent IL6/STAT3 signaling pathway in CCl 4 -induced rat liver fibrosis

doi: 10.1371/journal.pone.0206130

Figure Lengend Snippet: Sequence of primers used in RT-qPCR analysis.

Article Snippet: Slides were washed with TBS-Triton three times and one time with TBS for 5 min. Nonspecific binding was blocked using 5% goat serum for 30 min and the blocking buffer was then removed and sections were incubated with a polyclonal anti–IL17A antibody (1:2000) (USBiological, Salem, MA, USA), polyclonal anti–IL17RA antibody (2.5μg/ml) (GeneTex, Irvine, CA, USA) and polyclonal antibody to PCNA (2.5mg/ml) (ThermoFisher Scientific, Rockford, IL, USA).

Techniques: Sequencing

Journal: PLoS ONE

Article Title: Bone marrow derived-mesenchymal stem cells downregulate IL17A dependent IL6/STAT3 signaling pathway in CCl 4 -induced rat liver fibrosis

doi: 10.1371/journal.pone.0206130

Figure Lengend Snippet: The data are presented as fold induction of mRNA expression of a . Il17a , b . Il17f , c . Il17ra , and d . Il17rc in CCl 4 /untreated (n = 5) rats compared to MSCs transplanted (CCl 4 +BM-MSCs) groups (n = 5) after 2, 4 and 6 weeks. All groups are compared with normal/diet control and oil/vehicle control rats (n = 5). Each experiment was performed in duplicates. Representative data are shown as mean±SD. * P <0.05. # P <0.05 compared to control groups.

Article Snippet: Slides were washed with TBS-Triton three times and one time with TBS for 5 min. Nonspecific binding was blocked using 5% goat serum for 30 min and the blocking buffer was then removed and sections were incubated with a polyclonal anti–IL17A antibody (1:2000) (USBiological, Salem, MA, USA), polyclonal anti–IL17RA antibody (2.5μg/ml) (GeneTex, Irvine, CA, USA) and polyclonal antibody to PCNA (2.5mg/ml) (ThermoFisher Scientific, Rockford, IL, USA).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Bone marrow derived-mesenchymal stem cells downregulate IL17A dependent IL6/STAT3 signaling pathway in CCl 4 -induced rat liver fibrosis

doi: 10.1371/journal.pone.0206130

Figure Lengend Snippet: Upper panels are representatives of IL17 expression and it is noted that it was very weak in normal and paraffin/oil control group, severe in CCl 4 /fibrosis and recovery groups, high in CCl 4 +BM-MSCs/2 weeks group, moderate in CCl 4 +BM-MSCs/4weeks group and mild CCl 4 +BM-MSCs/6 weeks group. Lower panels are representatives of IL17RA expression and it is noted that it was absent in normal and paraffin/oil control group, high in CCl 4 /fibrosis and recovery groups, moderate in CCl 4 +BM-MSCs/2weeks group, mild in CCl 4 +BM-MSCs/4weeks group and nil in CCl 4 +BM-MSCs/6 weeks group (400X).

Article Snippet: Slides were washed with TBS-Triton three times and one time with TBS for 5 min. Nonspecific binding was blocked using 5% goat serum for 30 min and the blocking buffer was then removed and sections were incubated with a polyclonal anti–IL17A antibody (1:2000) (USBiological, Salem, MA, USA), polyclonal anti–IL17RA antibody (2.5μg/ml) (GeneTex, Irvine, CA, USA) and polyclonal antibody to PCNA (2.5mg/ml) (ThermoFisher Scientific, Rockford, IL, USA).

Techniques: Expressing